Genetic variability of Grapevine Leafroll-Associated Virus-1,2,3,4 Infecting Vineyards in Tokat Province of Turkey

Grapevine leafroll disease (GLRD) is one of the most important viral diseases grapevine worldwide. The aim this study was to investigate prevelance associated virus-1 (GLRaV-1), leafroll-associated virus-2 (GLRaV-2), virus-3 (GLRaV-3), virus 4 (GLRaV-4), and virus-9 (GLRaV-9) in Tokat province Turkey. In 2018, leaf samples one-year-old shoots plants, showing virus-like symptoms, were collected from different vineyards Central, Erbaa, Niksar, Pazar districts province. Totally 197 obtained tested molecularly using two-step polymerase chain reaction (PCR) by virus-specific primers for GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4) GLRaV-9. At least 106 (53.80%) found be infected showed single or multiple infections. 103 (52.28%) resulted positive with GLRaV-1 while infection rates %5.18 GLRaV-3 10 samples, %2.53 GLRaV-4 5 %1.03 GLRaV-2 GLRaV-9 2 respectively. amplified PCR products sequenced, aligned MEGAX, analyzed BLAST comparisons other isolates GenBank. Sequence analysis viruses 94-96 % , 96-99 and, 99-100 nucleotide identity references GenBank UO3-3 isolate